ABSTRACT
Objective To explore the sensitivity and specificity of anti‐cyclic citrullinated peptide antibody (anti‐CCP antibody) and anti‐keratin antibody (anti‐AKA antibody) detection for diagnosing rheumatoid arthritis (RA) and the application value of their combination detection in the diagnosis and treatment of RA .Methods The chemiluminescent microparticle immunoassay and indi‐rect immunofluorescence analysis were adopted to detect anti‐CCP antibody and anti‐AKA antibody in 80 cases of RA ,40 cases of other auto‐immune diseases(AID) and 30 people undergoing the physical examination .Results The positive rates of anti‐CCP anti‐body and anti‐AKA antibody in the RA group were significantly higher than those in the non‐RA group and control group ,the differences were statistically significant (P< 0 .05) .The sensitivity of single anti‐CCP antibody detection in the RA group was 73 .8% ,which was higher than 41 .3% of anti‐AKA antibody ,while the specificity of single anti‐CCP antibody detection was 86 .2% ,which was lower than 95 .7% of anti‐AKA antibody detection .In their combined detection ,the sensitivity and specificity were increased .Conclusion The anti‐CCP antibody detection has higher sensitivity and specificity for diagnosing RA .Its combined detection with anti‐AKA antibody can avoid the missed diagnosis of atypical clinical early stage RA ,which has a higher clinical value in the diagnosis and prognosis of RA .
ABSTRACT
Objective To analyze the distribution of pathogens isolated from medistream urine specimens of patients with urinary infection and their antibacterial susceptibility ,so as to provide references for rational use of antibacterial agents .Methods A total of 853 medistream urine specimens collected from January to December 2014 in this hospital were cultured ,bacterial identification and antimicrobial susceptibility testing were carried in the isolates .Results Pathogens were isolated from 245 medistream urine specimens ,and the positive rate was 28 .7% .A total of 269 strains were isolated and most were gram‐negative bacteria(accounted for 60 .2% ) ,followed with gram‐positive bacteria(accounted for 33 .8 % ) and fungi(accounted for 5 .9% ) .A total of 48 strains of extended‐spectrumβ‐lactamases(ESBLs)producing Escherichia coli and 3 strains of ESBLs producing Klebsiella pneumoniae were i‐solated .The detection rate of methicillin resistant Staphylococcus epidermidis (MRSE) and methicillin resistant Staphylococcus au‐reus(MRSA) was 17 .6% and 35 .8% ,respectively .Conclusion Clinical physicians should scientifically and rationally select anti‐bacterial drugs according to drug‐sensitivity results and avoid blindly empirical use of antibacterial agents ,in order to reducing and controlling the emergence and prevalence of ESBLs producing strains and other drug‐resistant bacteria .